Cross-Hybridization Analysis Network of Gene Expression
 --  Cross-Hybridization Based Hybridization Probe Design

Species

Homo sapiens
Mus musculus
Saccharomyces cerevisiae

Input UniGene cluster ID (such as Hs.1311), gene symbol (such as CD1C), or yeast ORF (YAL007C):


Primer Picking Options

Primer Number to Return:

Product Size: Min: Opt: Max:

Primer Size: Min: Opt: Max:

Primer Tm: Min: Opt: Max:

Primer GC%: Min: Opt: Max:

Cross-Hybridization Options

Will be considered as cross-hybridization if the following criteria are met:

Minimal percent identity: %

Minimal length of similar fragment:

This tool masks the cross-hybridized region in Primer3 analysis. Primer3 is written by Steve Rozen and Helen J. Skaletsky.