EST Projects at YMU

Vector List

1. pCRII

2. pCRII-TOPO

3. pTripIEx2

4. pGEM-T Easy

pCRII

NAME : pCRII
SOURCE : Invitrogen
VECTOR SEQUENCE : Avaliable here
VECTOR MAP : Avaliable here
V_TYPE : Plasmid
RE_1 : Nsi I
RE_2 : Hind III
PRIMER_NAME :

          Nested PCR primer 1 5'-  TCGAGCGGCCGCCCGGGCAGGT-3' 
          Nested PCR primer 2R 5'-AGCGTGGTCGCGGCCGAGGT-3'
SEQ_PRIMER: T7 or SP6

pCRII-TOPO

NAME : pCRII -TOPO
SOURCE : Invitrogen
VECTOR SEQUENCE : Avaliable here
VECTOR MAP : Avaliable here
V_TYPE : Plasmid
RE_1 : Nsi I
RE_2 : Hind III
PRIMER_NAME :

          Nested PCR primer 1    5'-TCGAGCGGCCGCCCGGGCAGGT-3'
          Nested PCR primer 2R 5'-AGCGTGGTCGCGGCCGAGGT-3'
SEQ_PRIMER : T7 or SP6

pTripIEx2

NAME : pTripIEx2
SOURCE : Clontech
VECTOR SEQUENCE : Avaliable here
VECTOR MAP : Avaliable here
V_TYPE : phagemid
RE_1 : Sfi I
PRIMER_NAME : TripEx
SEQ_PRIMER :

          TripEx5': 5'- CTC GGG AAG CGC GCC ATT GTG TTG G-3'
          TripEx3': 5'- ATACGA CTC ACT ATA GGG CGA ATT GGC -3'

pGEM-T Easy

NAME : pGEM-T Easy
SOURCE : Promega
VECTOR SEQUENCE : Avaliable here
VECTOR MAP : Avaliable here
V_TYPE : Plasmid
RE_1 : EcoR1

RE_2 : EcoR1
PRIMER_NAME : T7 or SP6
SEQ_PRIMER :

          Nested PCR primer 1    5'-TCGAGCGGCCGCCCGGGCAGGT-3'
          Nested PCR primer 2R 5'-AGCGTGGTCGCGGCCGAGGT-3'